Performance Criteria for Biosafety Cabinet

The European Standard specified basic requirements for Biosafety Cabinets with respect to safety and hygiene. The European Standard sets the minimum performance criteria for safety cabinets for work with microorganism and specifies test procedures for microbiological safety cabinets with respect to protection of the worker and the environment, product protection and cross contamination.

The terms Biosafety Cabinet should only be applied to those devices that meet the requirements of Class I, II, or III specifications, based on their construction, airflow velocities and patterns, and their exhaust systems.   

Major International Standards for Biological Safety Cabinets:

• European Standard EN 12469                                            • American Standard NSF 49

• Australian Standard AS 2252                                             • Japanese Standard JIS K 3800

Features	CLASS I	CLASS II TYPE A1	CLASS II TYPE A2	CLASS II TYPE B1	CLASS II TYPE B2	CLASS II
Inflow Velocity (m/s)	0.70	0.40	0.40	0.40	--	--
Recycle Air (%)	0	70	70	30	0	0
Exhaust Air (%)	100	30	30	70	100	100
Control Plenum Surrounded by	Outside    Air	Outside Air	Negative Plenum	Negative Plenum	Negative Plenum	Negative Plenum
Exhaust Alternatives	Inside room/Hard duct	Inside room/Thimble duct	Inside room/Thimble duct	Hard Duct only	Hard Duct only	Inside room/Hard duct
Biosafety Levels	1, 2 & 3	1, 2 & 3	1, 2 & 3	1, 2 & 3	1, 2 & 3	1, 2, 3 & 4

WAIOMETRA Class II Biosafety Cabinets

 

Waiometra Class II Biosafety Cabinets are a vertical laminar (unidirectional) HEPA-filtered air stream that descends downward from the interior of the cabinet. This continuously flushes the cabinet interior of airborne contaminants and protects samples being handled within the cabinet from contamination and is known as the downflow.

Class II Biosafety Cabinets protect both the operator and environment from exposure to biohazards. In addition, Class II cabinets also protect product samples from contamination during microbiological manipulations within the cabinet interior and are all suitable for work with agents assigned to biosafety levels 1, 2 and 3. 

WAIOMETRA Class III Biological Safety Cabinets

Waiometra Class III biosafety cabinet provides an absolute level of safety, which cannot be attained with Class I and Class II cabinets. All Class III cabinets are usually of welded metal construction and are designed to be gas tight. Work is performed through glove ports in the front of the cabinet. During routine operation, negative pressure relative to the ambient environment is maintained within the cabinet. This provides an additional fail-safe mechanism in case physical containment is compromised.

All Class III biosafety cabinets are suitable for work with microbiological agents assigned to biosafety levels 1, 2, 3 and 4. They are frequently specified for work involving the most lethal biological hazards.

Waiometra Biosafety Cabinet Class II and Class III are available in different sizes with duet & without duet.

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